Location: Bowdoin / Calendar

Weekly Chemistry Seminar-Matt Bogyo, Stanford University

  • 2/8/2013 | 3:00 PM – 4:00 PM
  • Location: Druckenmiller Hall, Room 020
  • Event Type: Seminar

Weekly Chemistry Seminar-Matt Bogyo, Stanford UniversityProteases are enzymes that primarily function by degrading protein substrates. Since this process is irreversible, proteases must be carefully regulated within cells and organisms in order to prevent undesired consequences. Furthermore, proteases often pathogenic roles in common human diseases such as cancer, asthma, arthritis and atherosclerosis. Over the past decade, my laboratory has developed a series of small molecule probes that specifically bind to the active form of protease targets through an enzyme catalyzed chemical reaction. These reagents freely penetrate cells and can be used to enrich complex proteomic samples for monitoring of global patterns of protease activity as well as to directly image protease activity in live cells and whole animals. They can also be used to monitor the efficacy and selectivity of small molecule protease inhibitor drugs. We are currently applying these probes to study the role of specific proteases in the process of angiogenesis and metastasis in mouse models of cancer as well as during the process of inflammation in mouse models of atherosclerosis and asthma. In addition we have developed probes that bind proteases associated with the process of programmed cell death (apoptosis). These reagents allow the direct non-invasive imaging of cell death in vivo. We are currently developing these tools to further study the cell biology of tumor response to chemotherapy. Recent advances in these projects will be presented