Location: Bowdoin / Anne McBride


Anne McBride

Associate Professor of Biology and Biochemistry
(on leave for the 2016-17 academic year)

Contact Information


Druckenmiller Hall - 204B

Anne McBride


The addition of different chemical groups to amino acids within proteins can influence how proteins function in an organism. Protein arginine methyltransferases are a family of eukaryotic enzymes that catalyze one of the smallest protein modifications, the addition of one or two methyl groups to the amino acid arginine. Arginine methylation of proteins affects many important cellular processes including gene expression, intracellular transport and signaling. The goal of research in the McBride lab is to understand different roles of this ubiquitous modification, using yeast as a model system.

By studying baker's yeast, we have been able to take advantage of many biochemical, genetic, genomic and cell biological tools to understand how arginine methylation affects protein transport between the nucleus and the cytoplasm of this single-celled eukaryote. We have now expanded these studies to the related yeast Candida albicans in the hopes of gaining insight into how arginine methylation affects protein function in this pathogenic yeast.

Ariyachet C*, Solis NV, Liu Y, Prasadarao NV, Filler SG, McBride AE. (2013) SR-like RNA-binding protein Slr1 affects Candida albicans filamentation and virulence. Infect Immun. 2013 Feb 4. [Epub ahead of print]

Young BD, Weiss DI, Zurita-Lopez CI, Webb KJ, Clarke SG, McBride AE. (2012) "Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation."  Biochemistry. 51(25):5091-104. 

McBride AE, Conboy AK*, Brown SP*, Ariyachet C*, Rutledge KL. (2009) "Specific sequences within arginine-glycine-rich domains affect mRNA-binding protein function." Nucleic Acids Res. 37(13):4322-30. 

McBride, AE, Zurita-Lopez, C, Regis, A, Blum, E, Conboy, A*, Elf, S* and Clarke, S (2007) “Protein arginine methylation in Candida albicans: role in nuclear transport.Eukaryot Cell. 6(7): 1119-29. 

McBride, AE (2006) "Diverse roles of protein arginine methyltransferases” in “Protein Methyltransferases" The Enzymes Vol. XXIV, ed. S. Clarke and F. Tamanoi, Academic Press, pp. 51-103.

McBride AE, Cook JT*, Stemmler EA, Rutledge KL, McGrath KA* and Rubens JA* (2005) "Arginine methylation of yeast mRNA-binding protein Npl3 directly affects its function, nuclear export, and intranuclear protein interactions." J Biol Chem 280(35):30888-98.

Yu MC, Bachand F, McBride AE, Komili S, Casolari JM and Silver PA (2004) "Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors." Genes Dev 18(16):2024-35.

*Bowdoin students


Tee Ariyachet

Posttranslational modification of a novel arginine/glycine rich protein in Candida albicans
(Spring 2009-Spring 2010)

Chris Carlin

Investigation of a putative arginine demethylase in the opportunistic commensal Candida albicans 
(Summer 2008-Spring 2009)

Hayley McHugh

Candida albicans Orf19.216 protein and its relationship with fluconazole resistance 
(Summer 2008-Spring 2009)

Yasmine White

Identification of putative arginine methyltransferases in S. cerevisiae and associated phenotypes
(Spring 2009)


Tee Ariyachet

Analysis of exoribonuclease Kem1 localization in Candida albicans
(Summer 2007)

Testing the function of the putative docking motif in the localization of Npl3 in Saccharomyces cerevisiae
(Spring 2008)

Exploring the function of hydrophobic residues in the arginine-glycine-rich (RG) domain of the RNA-binding protein Npl3
(Summer 2008) 

Lincoln Pac

Exploring the role of delta-nitrogen arginine methylation in yeast translation
(Fall 2007-Spring 2008)

Anthony Regis

Exploring the RNA-binding protein Npl3 and exoribonuclease Kem1: Intracellular localization and arginine methylation in Candida albicans
(Summer 2006-Spring 2007)

Alex Wilkinson

Identification of a methylated protein in Candida albicans
(Summer 2006, Summer 2007-Spring 2008)


Charlie Johnson

Insertion of an HA tag in HRP1 and THP2: investigation of protein-protein interactions.
(Fall 2005)

Kelly McGrath

Mutational analysis of an arginine-rich RNA-binding protein
(Summer 2004)
Effects of Arginine Methylation on Intranuclear Protein Interactions of RNA-Binding Proteins
(Fall 2005-Spring 2006)

Hilarie Wilson

Mapping sites of protein methylation by immunoblot analysis
(Summer 2004)


Emily Blum

Phenotypic analysis of an arginine methyltransferase (HMT1) deletion in Candida albicans
(Spring 2003-Spring 2004)

Shanique Brown

Investigating the effects of domain deletions on the multimerization of Npl3p in Saccharomyces cerevisiae
(Fall 2003-Spring 2004)

Jeff Cook

Arginine methylation of an RNA-binding protein
(Summer 2002, Spring 2003-Spring 2004)

Ana Conboy

Testing function of arginine-rich domains in baker's yeast
(Summer 2003)

Jeffrey Rubens

Genetic studies of the relationship between arginine methylation and RNA-binding protein function in yeast
(Fall 2002-Spring 2003)

Caroline Agusti

Analysis of the importance of protein arginine methylation in a fungal pathogen
(Fall 2002)

Shannon Elf

Arginine methyltransferase function in Candida albicans
(Summer 2002)


Michael Leibowitz

Analysis of interactions between an arginine methyltransferase and glycogen synthase kinase-3 family members in Saccharomyces cerevisiae
(Fall 2001-Spring 2002)

Tessie Ng

Structural and functional comparison of nuclear transport factors in yeast
(Fall 2001-Spring 2002)

Kathryn Penney

Genetic analysis of the role of Rmt2, a novel arginine methyltransferase, in Saccharomyces cerevisiae
(Fall 2001-Spring 2002)

a protein containing rmt2