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Jennifer Kohler Seminar " Capturing glycoconjugate complexes with metabolically incorporated photo-sugars."

  • 9/27/2013 | 3:00 PM – 4:00 PM
  • Location: Druckenmiller Hall, Room 020
  • Event Type: Lecture

Jennifer Kohler Seminar " Capturing glycoconjugate complexes with metabolically incorporated photo-sugars."Glycan-mediated interactions constitute the underlying molecular bases for a wide range of biological processes.  This class of interactions is particularly important in development, immunology, infection, and carcinogenesis.  Yet glycan-mediated interactions are difficult to detect and characterize, due to their low affinities and rapid dissociation kinetics.  To capture these ephemeral complexes, we make use of metabolic oligosaccharide engineering to incorporate photocrosslinking groups into cellular glycoconjugates.  In this presentation, I will describe our strategy for introducing photocrosslinkers into a variety of glycoconjugates, how we characterize which glycoconjugates are modified and the degree of modification, and the use of these photocrosslinkers to discover glycan-mediated interactions.  The utility of this approach will be demonstrated by describing the discovery of a novel receptor for cholera toxin.