Applications in Spectrophotometry
Day 1
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| Morning Session |
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- Introductions
- Introduction to Spectrophotometry
- Lab - Absorption spectra of food colorings, chromatography and spectra of Kool-aid and mouthwashes.
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| Afternoon Session |
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- Background on standard curves, Beer-Lambert law
- Lab - creating standard curves, identifying food dyes, forensic lab
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Day 2
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| Morning Session |
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- Background - enzyme kinetics
- Lab - Protein stand curve and assay, determination of protein content in beverages, glucose standard curve.
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| Afternoon Session |
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- Lab - enzyme assays: glucose trinder, ONPG, Lactaid (b-galactosidase), Beano (a-galactosidase)
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Day 3
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| Morning Session |
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- Background - photosynthesis and membrane structure
- Lab - Plant pigments: separation and identification, membrane structure (beets)
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| Afternoon Session |
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- Background - water quality
- Lab - water quality: phosphate and nitrate analysis, chlorine levels in swimming pools
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Day 4
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| Morning Session |
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- Background - bacterial growth
- Lab - bacteria growth curved, set-up for lab experience with Upward Bound students
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| Afternoon Session |
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- Lab with Upward Bound students
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Day 5
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| Morning Session |
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- Lab - complete bacteria growth experiment, perform other experiments of interest (sunscreen analysis, bleach kinetics, olive oil analysis, among others)
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| Afternoon Session |
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- Pack-up materials for transport home, evaluations
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Home • 2008 Workshop Agenda • Past Participants • Lab Descriptions
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Please send questions, comments, and suggestions to Pam Bryer .
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Howard Hughes Medical Institute and Bowdoin College
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